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wash buffer  (Novus Biologicals)


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    Structured Review

    Novus Biologicals wash buffer
    Wash Buffer, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+c/c-Myc+Antibody+(Myc%2EA7)+-+Azide+and+BSA+Free/us12612461-525-6-16
    Average 93 stars, based on 10 article reviews
    wash buffer - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    FACS:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, 10 μl of clone lysate was added to 90 μl of Fc blocked U937 (2.5×10{circumflex over ( )}5) in FACS buffer, incubated for 30 min at 4° C., washed twice with FACS buffer by spinning at 500G for 2 min, a-cMyc-PE (Novus Cat. #NB200-108PE, 1:200 dilution) was added to the cells, incubated for 30 min at 4° C., washed twice with FACS buffer by spinning at 500G for 2 min, fixed with 1% PFA in PBS, then analyzed by flow cytometry. ..

    Incubation:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, 10 μl of clone lysate was added to 90 μl of Fc blocked U937 (2.5×10{circumflex over ( )}5) in FACS buffer, incubated for 30 min at 4° C., washed twice with FACS buffer by spinning at 500G for 2 min, a-cMyc-PE (Novus Cat. #NB200-108PE, 1:200 dilution) was added to the cells, incubated for 30 min at 4° C., washed twice with FACS buffer by spinning at 500G for 2 min, fixed with 1% PFA in PBS, then analyzed by flow cytometry. ..

    Article Title: Transcription factor NFYA directs male meiotic entry by regulating accessible chromatin at meiotic promoters in mice
    Article Snippet: Dried slides were washed at room temperature for 10 min in a step-wise manner with (i) 1× PBS containing 0.4% (v/v) Photo-Flo 200 (Kodak; 1026269), (ii) 1× PBS containing 0.1% (v/v) Triton X-100, (iii) ADB/PBS solution [10% ADB (0.03% (w/v) BSA, 0.01% (v/v) Triton X-100, 10% (v/v) Donkey serum (Sigma, D9663), 2× PBS) and 90% 1× PBS). .. Slides were then incubated at 4 °C overnight with primary antibodies diluted in ADB solution (anti-γH2AX, Novus Biologicals, NB100-384, 1:1000 dilution; anti-SCP-3 (D-1), Santa Cruz, sc-74569, 1:400 dilution; anti-GCNA, Abcam, ab82527 1:1000 dilution; anti-DMRT1, Santa Cruz, sc-377167, 1:200 dilution). .. The next day, slides were washed sequentially as (i–iii, above), and incubated at 37 °C for 1 h with secondary antibodies diluted 1:2,000 in ADB (Alexa Fluor anti-mouse 594, Thermo Fischer Scientific, A21203; Alexa Fluor anti-rabbit 488, Thermo Fisher Scientific, A21206; Alexa Fluor anti-rat 594, Thermo Fisher Scientific, A21209).

    Article Title: Glutamate Carboxypeptidase II (GCPII)-Targeted PET to Identify Muscle Denervation in Peripheral Nervous System Injuries
    Article Snippet: Proteins were transferred onto PVDF membranes using a Trans-Blot Turbo system (Bio-Rad, 1704156EDU) at 1 A and 25 V for 30 minutes. .. Membranes were blocked for 5 minutes at room temperature in EveryBlot blocking buffer (Bio-Rad, cat. no. 12010020) and then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (mouse anti-GCPII, 1:500, Novus Biologicals, cat. no. NBP1-45057; rabbit anti-GAPDH (14C10), 1:30,000, Cell Signaling Technology, cat. no. 2118S). .. Membranes were washed with TBS-T (four quick washes followed by five, five-minute washes) and incubated with HRP-conjugated secondary antibodies (goat anti-mouse (H+L), 1:2,000, Invitrogen, cat. no. G21040; goat anti-rabbit, 1:3,000, Sigma-Aldrich, cat. no. 12-348) for 1 hour at room temperature.

    Article Title: Transcription factor NFYA directs male meiotic entry by regulating accessible chromatin at meiotic promoters in mice.
    Article Snippet: Dried slides were washed at room temperature for 10 min in a step-wise manner with (i) 1× PBS containing 0.4% (v/v) Photo-Flo 200 (Kodak; 1026269), (ii) 1× PBS containing 0.1% (v/v) Triton X-100, (iii) ADB/PBS solution [10% ADB (0.03% (w/v) BSA, 0.01% (v/v) © The Author(s) The EMBO Journal 19 Triton X-100, 10% (v/v) Donkey serum (Sigma, D9663), 2× PBS) and 90% 1× PBS). .. Slides were then incubated at 4 °C overnight with primary antibodies diluted in ADB solution (anti-γH2AX, Novus Biologicals, NB100-384, 1:1000 dilution; anti-SCP-3 (D-1), Santa Cruz, sc-74569, 1:400 dilution; anti-GCNA, Abcam, ab82527 1:1000 dilution; anti-DMRT1, Santa Cruz, sc-377167, 1:200 dilution). .. The next day, slides were washed sequentially as (i–iii, above), and incubated at 37 °C for 1 h with secondary antibodies diluted 1:2,000 in ADB (Alexa Fluor anti-mouse 594, Thermo Fischer Scientific, A21203; Alexa Fluor anti-rabbit 488, Thermo Fisher Scientific, A21206; Alexa Fluor anti-rat 594, Thermo Fisher Scientific, A21209).

    Article Title: KCTD10 weakens m6A modification of IL-11 in a ubiquitin-dependent manner to repress tumour immune escape in lung adenocarcinoma.
    Article Snippet: Oncology Department, The Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, Zhejiang, China Department of Respiratory, The Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, Zhejiang, China Academician Expert Workstation, Zhejiang Luoxi Medical Technology Co., Ltd., Hangzhou, Zhejiang, China Zhejiang Luoxi Medical Technology Co., Ltd., Hangzhou, Zhejiang, China

    Flow Cytometry:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, 10 μl of clone lysate was added to 90 μl of Fc blocked U937 (2.5×10{circumflex over ( )}5) in FACS buffer, incubated for 30 min at 4° C., washed twice with FACS buffer by spinning at 500G for 2 min, a-cMyc-PE (Novus Cat. #NB200-108PE, 1:200 dilution) was added to the cells, incubated for 30 min at 4° C., washed twice with FACS buffer by spinning at 500G for 2 min, fixed with 1% PFA in PBS, then analyzed by flow cytometry. ..

    Blocking Assay:

    Article Title: Glutamate Carboxypeptidase II (GCPII)-Targeted PET to Identify Muscle Denervation in Peripheral Nervous System Injuries
    Article Snippet: Proteins were transferred onto PVDF membranes using a Trans-Blot Turbo system (Bio-Rad, 1704156EDU) at 1 A and 25 V for 30 minutes. .. Membranes were blocked for 5 minutes at room temperature in EveryBlot blocking buffer (Bio-Rad, cat. no. 12010020) and then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (mouse anti-GCPII, 1:500, Novus Biologicals, cat. no. NBP1-45057; rabbit anti-GAPDH (14C10), 1:30,000, Cell Signaling Technology, cat. no. 2118S). .. Membranes were washed with TBS-T (four quick washes followed by five, five-minute washes) and incubated with HRP-conjugated secondary antibodies (goat anti-mouse (H+L), 1:2,000, Invitrogen, cat. no. G21040; goat anti-rabbit, 1:3,000, Sigma-Aldrich, cat. no. 12-348) for 1 hour at room temperature.

    Article Title: KCTD10 weakens m6A modification of IL-11 in a ubiquitin-dependent manner to repress tumour immune escape in lung adenocarcinoma.
    Article Snippet: Oncology Department, The Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, Zhejiang, China Department of Respiratory, The Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, Zhejiang, China Academician Expert Workstation, Zhejiang Luoxi Medical Technology Co., Ltd., Hangzhou, Zhejiang, China Zhejiang Luoxi Medical Technology Co., Ltd., Hangzhou, Zhejiang, China

    Binding Assay:

    Article Title: Tumor cell death by ferroptosis contributes to an immunosuppressive tumor microenvironment in syngeneic murine models of cancer
    Article Snippet: .. Cells were washed once in ice-cold Annexin Binding Buffer (Invitrogen, V13246) then resuspended in ice-cold staining solutions containing Annexin V buffer plus Calreticulin-FITC (Novus Biologicals, NBP1-47518F) at a 1:100 dilution or Annexin V-Pacific Blue (Molecular Probes, A35122) at a 1:300 dilution. ..

    Article Title: Tumor cell death by ferroptosis contributes to an immunosuppressive tumor microenvironment in syngeneic murine models of cancer.
    Article Snippet: .. Cells were washed once in ice-cold Annexin Binding Buffer (Invitrogen, V13246), then resuspended in ice-cold staining solutions containing Annexin V buffer plus Calreticulin-FITC (Novus Biologicals, NBP1-47518F) at a 1:100 dilution or Annexin VPacific Blue (Molecular Probes, A35122) at a 1:300 dilution. ..

    Staining:

    Article Title: Tumor cell death by ferroptosis contributes to an immunosuppressive tumor microenvironment in syngeneic murine models of cancer
    Article Snippet: .. Cells were washed once in ice-cold Annexin Binding Buffer (Invitrogen, V13246) then resuspended in ice-cold staining solutions containing Annexin V buffer plus Calreticulin-FITC (Novus Biologicals, NBP1-47518F) at a 1:100 dilution or Annexin V-Pacific Blue (Molecular Probes, A35122) at a 1:300 dilution. ..

    Article Title: Tumor cell death by ferroptosis contributes to an immunosuppressive tumor microenvironment in syngeneic murine models of cancer.
    Article Snippet: .. Cells were washed once in ice-cold Annexin Binding Buffer (Invitrogen, V13246), then resuspended in ice-cold staining solutions containing Annexin V buffer plus Calreticulin-FITC (Novus Biologicals, NBP1-47518F) at a 1:100 dilution or Annexin VPacific Blue (Molecular Probes, A35122) at a 1:300 dilution. ..



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